Pemeriksaan ini biasanya dilakukan untuk menunjang diagnososa infeksi G0.
1. Preparat yang telah dibuat, didiamkan beberapa saat di udara, panaskan sebentar dan dinginkan.
2. Preparat posisi horizontal dituangi metylen blue sampai tergenang. Diamkan 1 menit. Cuci dengan air mengalir pada posisi vertikal sampai air tidak berwarna lagi.
3. Preparat posisi horizontal tuangi lugol 1% sampai tergenang. Diamkan 1 menit, cuci di air mengalir.
4. Tuangi air fuchsin lindi 0,35%. Biarkan 30 detik. Cuci.
5. Tegakkan objek glass, keringkan pada udara terbuka.
6. Periksa dibawah mikroskop dengan olie emersi.
Hasil: terlihat coccus berwarna biru tapi dapat dibedakan Diplococcus GO atau Diplococcus Gram (+).
Lihat:
- adanya diplokokus intra/ekstrasel.
- adanya leukosit dan jumlahnya LPB/LPK.
- adanya sel epitel dan jumlahnya LPB/LPK.
- adanya sekret berupa lendir, pada pemeriksaan tampak sebagai benang merah.
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8 years ago
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ReplyDeletedapet dari sumber mana yach???
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